Population Genetics Technologies Ltd.
- Available Intellectual Property Assets - 


PGT IP FAMILY

034



Method for Normalizing a Nucleic Acid Sample


DESCRIPTION

Covers an approach using Cas9 for normalization of a nucleic acid sample. Large numbers of samples of varying concentration can be pooled and subjected to the process with the result being a normalised pooled sample at the concentration desired.

APPLICATIONS

  • Any application where normalisation is required.
  • Ideally suited for samples pooled prior to next-gen sequencing (NGS), replacing the need for individual sample quantitation/selective volume use prior to pooling.

KEY IP

  • GB patent application filed. WO/PCT to be filed and at National Phase will be progressed including US and Europe filings.
  • Application available for review under appropriate confidentiality agreement with PGT.


PGT IP FAMILY

033



Method for Fragmenting a Nucleic Acid for Sequencing


DESCRIPTION

Covers DNA fragmentation employing methylation-specific restriction enzymes that enable simultaneous fragmentation and adapter-labelling.

APPLICATIONS

  • Any application requiring DNA fragmentation, such as those using next-gen sequencing (NGS).
  • Highly controllable, robust and low-cost alternative to existing fragmentation-adaptor ligation approaches.

KEY IP

1 GB patent issued; WO/PCT at National Phase has been progressed to US and Europe filings.


PGT IP FAMILY

031



Method for Identifying Minority Variants in a Sample


DESCRIPTION

Covers a workflow which enables accurate and sensitive determination of sequence variation in the nucleic acids in a sample, even when present in a wide concentration range. Achieved via sampling and amplifying a small proportion (bottleneck) of the initial amplified, fragmented sample(s).

APPLICATIONS

Generally applicable to any nucleic acid source but ideally suited for applications with RNA starting points, e.g. HIV or HCV viral genomes which exhibit a high dynamic range in clinical samples.

KEY IP

US and EP patent patents pending (published; under review)



PGT IP FAMILY

024


Methods and Compositions for Polynucleotide Library Production, Immortalization and Region of Interest Extraction


DESCRIPTION 

Covers a method for highly multiplexed targeted sequencing from one or more samples via a circularised library.

Applications

  • Targeted sequencing on single or pooled samples (retaining an ‘immortalised’ library for subsequent resampling in other genomic regions of interest).
  • High throughput screening tool for en masse identification of transgene or other insertion flanking sequences. 
  • Previously commercialised as ‘AmbiTag’ high throughput transgene flanking region sequencing:

Key IP

1 issued US patent, 1 issued EP patent validated in FR, DE and UK.


PGT IP FAMILY

016



Methods and Compositions for Isolating Nucleic Acid Sequence Variants


DESCRIPTION 

Patents cover

  1. Hybridisation based sorting of samples, and
  2. Elution of a specific polynucleotide population using a strand displacing polymerase and polynucleotide specific primers.

APPLICATIONS

  • Sorting of nucleic acid sub-populations, including use to detect nucleotide variation.
  • Isolation of specific hybridisation-captured polynucleotides via enzymatic elution.

KEY IP 

2 issued US patents.


PGT IP FAMILY

008



Selective Genome Amplification


DESCRIPTION 

Covers methods of RNA mediated amplification of nucleic acids on a solid support.

APPLICATIONS

  • Any application requiring amplification of selected polynucleotides.

KEY IP 

Issued US patent.


PGT IP FAMILY

007



Nucleic Acid Analysis Using Sequence Tokens


DESCRIPTION  

Patents cover sequence tagging (token tagging) and pooling concepts, as well as variant detection via a number of readout platforms including ones that are hybridisation-based.

APPLICATIONS

  • Any involving pooling of tagged samples which differ from one another in sequence.
  • DNA variant detection, including those via hybridisation methods.

KEY IP

3 issued US patents. Note differences in claims.


PGT IP FAMILY

002



Genetic Analysis by Sequence-Specific Sorting


Description

Covers base-by-base sorting for reducing the complexity of genomes, and for variant detection.

Applications

  • High accuracy variant detection – can be used in complex mixtures including sample pools of high degree of multiplexing (e.g., 1000s of samples).

KEY IP

2 issued US patents, 1 issued European patent.


PGT IP FAMILY

001



Multiplexed Analytical Platform


Description

Covers concepts and methods for converting nucleotide variation into a tagged sequence for readout using a solid phase detection. Early (2004) priority date and broad claims.

Applications

  • Variant detection via hybridisation readout.

Key IP

1 issued US patent.

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